By Nathan P. Kaplan, Nathan P. Colowick, C. H.W. Hirs, Serge N. Timasheff
The significantly acclaimed laboratory typical, Methods in Enzymology, is among the so much hugely revered guides within the box of biochemistry. given that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate this day - actually an important ebook for researchers in all fields of existence sciences
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Extra resources for Enzyme Structure Part I
R. Benson and P. E. Hare, Proc. Natl. Acad. Sci. A. 7 2 , 6 1 9 (1975). s M. Weigele, S. deBernardo, and W. Leimgruber, Biochem. Biophys. Res. Commun. 50, 352 (1973). 4 p. A. St. , Silver Springs, Maryland) 31, 1 (1975). 5 j. Reiland and J. R. Benson, D u r r u m Resin Report No. , Palo Alto, California) (1976). 6 D. G. D r e s c h e r and K. S. Lee, Anal. Biochem. 84, 559 (1978). 7 E B/Shlen and M. Mellet, Anal. Biochem. 94, 313 (1979). METHODS IN ENZYMOLOGY, VOL. 91 Copyright © 1983by AcademicPress, Inc.
This situation may be exploited to suppress amino acid peaks that are only partially resolved from the imino acids to a degree where accurate integration of imino acid peaks without total separation becomes feasible. Roth a has investigated the use of another fluorescence reagent (7chloro-4-nitrobenzo-2-oxa-l,3-diazole, NBD chloride) for the determination of plasma imino acids after ion-exchange chromatography. Under typical analysis conditions as described above, this reagent forms fluorophors preferentially with imino acids.
It should be emphasized that the background data presented here should not be taken as a universal correction factor but rather as a model and the appropriate correction can only be obtained from each independent experiment. Background Contributors Normal precautions must be taken to provide for a "clean" experiment. Acid-washed glassware, electrophoretic grade reagents, and sterile gloves should be used at all times. In experiments described herein, amino acid analyses of reagents and buffers showed no detectable recovery of free amino acids.