By R. O. Morris, D. A. Regier, E. M. S. MacDonald (auth.), Professor Dr. Jean Guern, Professor Dr. Claude Péaud-Lenoël (eds.)
In a few contemporary biology conferences, plant progress components were a favourite subject. One may possibly ask if a brand new colloquium dedicated to the metab olism and the actions of cytokinins, thought of on the molecular point, used to be relatively beneficial and should upload something vital to the former contributions. in truth, the first goal of the organizers, of the French nationwide middle of medical study, and of the nationwide In stitute of Agronomic learn which backed the colloquium was once much less the version of a lawsuits e-book than the will to assemble a few scientists who had made contributions in the direction of fixing prob· lems in cytokinin examine and thereby to supply a chance for fruitful discussions with no regulations within the timetable. certainly this first aim used to be attained. in addition, vital new fmdings and con clusions have been provided via the individuals that merited ebook within the shape ofthis booklet. it truly is past the scope of this foreword to adver tize or perhaps to categorise those new fmdings. we will in basic terms point out some of them. The reader will get pleasure from the contributions of these authors who labored tough to clarify the biosynthetic pathways of common cytokin ins.
Read Online or Download Metabolism and Molecular Activities of Cytokinins: Proceedings of the International Colloquium of the Centre National de la Recherche Scientifique held at Gif-sur-Yvette (France) 2–6 September 1980 PDF
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Extra info for Metabolism and Molecular Activities of Cytokinins: Proceedings of the International Colloquium of the Centre National de la Recherche Scientifique held at Gif-sur-Yvette (France) 2–6 September 1980
A new synthesis oflabeled cytokinins has to be strongly prevented by the following chase with the WOO-fold concentration of [12 C]-adenine and the resulting dilution of the radioactive pool. The maximum radioactive amount of cytokinins should be found immediately after pulse ending and then should decrease logarithmically. Corresponding to our results, a maximum cytokinin content of 3000 cpm is expected to be reached a short time after pulse ending. Figure 5 shows the resulting curves which differ completely from the first ones.
Thus it appears that the marked increase in free i Ade in the culture medium which is associated with pathogenicity may result from a high rate of i 6 AMP production, catalyzed by cytokinin synthase. U 4 Plasmids All four pathogenic strains (MW2, Cf2, Cfl, Cfl5) contained a 63-megadalton (Mdal) plasmid, as estimated by agarose gel electrophoresis (Fig. 4). The first three most virulent strains contained an additional 120-Mdal plasmid. A third plasmid of 5 Mdal has been found in the Cfl and Cf2 strains.
0-----0 4 + 5'-nucleotidase . - - - . - 5 ' -nucleotidase ~ E Q. ' 2 I : G ~ I ~ , y;' .... ~. 0 -30 -20 -/0 ~ I. ~ 0 e " +/0 +20 +30 Strip number Fig. 3 A, B. Characterization of biosynthesized cytokinin nucleotide by -paper electrophoresis. A Electrophoretic analysis of radioactive cytokinin nucleotide (--) which was initially isolated from Dowex l-X4 column (Fig. 2B, F-a) and purified further by Sephadex LH-20 column; and isomerase treated [1_ 14 Cjf>3_iPP (0 .. 0). B 5'-nucleotidase treatment of the presumed of the presumed [ 14 Cli6 Ado-5'-P.